Experimental ulcers in the small intestine of rats:Part 2. Autoradiographical analysis of the healing processes of small intestinal ulcers

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• For the purpose of studying the healing process of ulcers, experimental ulcers were induced in the small intestine of rats, and one week later (acute stage) and 2 months afterward (chronic stage) 1μc/g body weight of<SUP>3</SUP>H-thymidine was injected into the peritoneal cavity of these and control rats for the autoradiographic analysis. One animal each from the acute, the chronic and the control groups was sacrificed at interval of one hour for 17 hours. The cell cycle of the epithelium of the small intestine was determined by calculating the percentage labeling of mitotic cells. Serial sections of the ulcer margin were prepared for reconstruction study of regenerating epithelium two hours after<SUP>3</SUP>H-thymidine injection. Then the proliferative capacity of smooth muscle cells of the ulcer margin and that of granulating tissues at the base of the ulcer were also studied two hours after the thymidineinjection.<BR>As a result it was found that the cell cycle of epithelium at the ulcer margin was significantly accelerated in the acute stage (GT=8.9 hr) and in the chronic stage (GT=9.0 hr) as compared with that in controls (GT=10.8 hr). These were mostly due to the shortening of G1 phase. However, it was demonstrated that the generation time of individual proliferating cells varied a great deal in the chronic stage. Study of the reconstruction model in the acute stage revealed hypertrophied and multilobulated crypts with poor thymidine intake in newly formed epithelium. On the other hand, in the chronic stage this histological feature became more prominent in association with marked thymidine intake up to the surface epithelium, and new crypts were generated from the surface epithelium and crypts at the ulcer margin themselves. Fairly many labeled nuclei of the smooth muscle cells at ulcer margin were found in the acute stage, but few in the chronic stage. A large number of labeled cells of granulating tissue at the base of the ulcer were observed in the acute stage, and a fairly large number in the chronic stage as well.

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財団法人 日本消化器病学会 | 論文

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