結核菌耐性検査培地の保存による薬剤力価の低下に関する研究
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概要
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In our country 1% Ogawas egg media are used for drug resistance test of tubercle bacilli. The report presents the results of experimental studies on decrease of drug acitivity during the storage of drug-containing media in diferent temperature.<BR>The methods of examination and the results of studies are summarized as follows:<BR>1) The drug-containing media of streptomycin, isoniazid, PAS, rifampicin, ethambutol, ethionamide, cycloserine, kanamycin, capreomycin, viomycin and enviomycin (a new drug produced in Japan and used for treatment of tuberculosis) were prepared and stored in 4-5°C refrigerator, 22°C, 30°C, 37°C thermoregulators. The drug resistance test of H<SUB>37</SUB>Rv and strains isolated from previously untreated patients were performed at 1, 2 weeks and 1, 2 months after preparation of drug-containing media. The results of test were compared with that of test used drug-containing media before storage (used immediately after preparation).<BR>The decrease of drug activity was most marked in ethionamide and rifampicin containing media stored outside of refrigerator and the decrease of drug activity was not observed in PAS, streptomycin, kanamycin, capreomycin, viomycin and enviomycin containing media. The degree of decrease of drug activity was as follows; rifampicin>ethionamide>cycloserine>isoniazid>ethambutol.<BR>In the case of storage in refrigerator ethionamide showed the decrease of drug activity after two months storage, but other drug did not show the decrease of drug activity within 3 to 6 months.<BR>2) The drug-containing media of streptomycin, kanamycin, capreomycin, viomycin and enviomycin were stored in 30°C thermoregulator and the drug resistance was examined by using these media after 3 and 6 months storage. According to the test results, slight decrease of drug activity of streptomycin and capreomycin was observed but other drugs did not show the decrease of drug activity within 6 months.<BR>Conclusively, the decrease of drug activity was marked in ethionamide and rifampicin containing media and was slight in ethambutol containing media during storage outside the refrigerator. The drug-containing media for drug resistance test of tubercle bacilli should be stored in refrigerator afer preparation and be used within 1 month.
- 日本結核病学会の論文