SYNERGIC ACTIVITY OF LYSOZYME IN COMBINATION WITH ANTITUBERCULOUS AGENTS:Report I. Inhibitory Activity against M. Tuberculosis in vitro and Synergic Effect in Vivo Measured by S.S.A.A.T. Method

概要

論文の詳細を見る
Experimental studies were made to evaluate the influences of lysozyme, a mucopolysaccha ridase, upon Mycobacterium tuberculosis as well as to investigate its synergic actions with drugs for tuberculosis. The results obtained are summerized as follows: (1) The minimum inhibitory concentration of lysozyme in vitro for the strains of H37Rv, R-INH and R-SM were found to be 400mcg/ml, 400mcg/ml and 200 mcg/ml, respectively. (2) The experiments on synergic actions of lysozyme with streptomycin, PAS or INH revealed that its synergic actions were quite evident both when lysozyme was adjunctively used with streptomycin against H37Rv strain and when it was used with SM or PAS against R-INH strain. (3) The addition of lysozyme to the incubated test tubes of M. tuberculosis resulted in the significant shrinkage and lysis of bacterial cells. This suggests that lysozyme possesses a potent bacteriolytic capacity. (4) In S.S.A.A.T. (specific serum antimycobacterial activity test) studies using rabbits, the synergic actions of lysozyme with kanamycin, streptomycin or INH against M. tuberculosis were observed to some extent.
一般社団法人日本結核病学会の論文