自然界抗酸菌に関する研究 : 1. 土壌抗酸菌の分離同定方法について

概要

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Acid-fast organisms were isolated from 240 soil samples collected at two areas in Japan and Korea. The biological and biochemical characteristics of the isolated mycobacteria were tested and compared with those of reference strains. The isolation rate and distribution of species of mycobacteria from soil were compared on three kinds of media with special reference to two different methods of isolation as well as different geographical areas. The results are summarized as follows: 1. Acid-fast bacilli were isolated most frequently on the 1% Ogawa medium with pretreat ment of soil samples by the Sohngens method. 2. No demonstrable difference was observed on the isolation rate between the soil from Hiroshima area and Seoul area. 3. As for Runyons grouping, 82.6% of the isolates from Hiroshima and 75.4% from Seoul were rapid growers. Scotochromogens were isolated 9.6% and 12.3%, and nonphotochromogens 4.5% and 8.2%, respectively. No photochromogens were isolated. 4. Three hundred and thirty-six strains were collected from the 240 soil samples. Neither tubercle bacilli nor M. avium-intracellulare complex were identified. The majority was M. fortuitum regardless of the area. The slow growers, especially M. scrofulaceum, were identified much more in Seoul than in Hiroshima, but the strain M. chelonei subsp. chelonei which was obtained in Hiroshima was not observed in Seoul.
日本結核病学会の論文