患者より分離した非定型抗酸菌(桜井株)によるマウス感染症に関する研究
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概要
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A strain of mycobacteria (Sakurai strain) was isolated from a patient and was classified into non-photochromogenic group. This strain was incidentally revealed by dilution method in vitro to be sensitive to cephaloridine (CER). The present study was undertaken to evaluate the efficacy of CER in vivo by administrating it to mice inoculated both with Sakurai strain and with two known acid-fast bacilli. During the course of this experiment, one group of mice showed a certain histological change of renal tubules, which was similar to that previously described by Atkinson et al. as a toxic effect of CER.<BR>(1) The Sakurai strain of atypical acid-fast bacilli was resistant to most of the known anti-tuberculosis agents, but was revealed as sensitive to CER by the disc method. The CER sensitivity examined by Kirchners liquid media showed partial inhibition with 12.5 mcg/ml and complete inhibition with 50 mcg/ml of CER.<BR>(2) In order to compare the virul ence of Sakurai strain with that of other known strain of tubercle bacilli, hybrid mice of ddy strain were divided into three groups, and group 1 was inoculated with strongly virulent Kurono strain (human type), group 2 with BCG and group 3with Sakurai strain. A part of the last group was administered with daily dose of 250 mg/kg of either CER or cephalothin (CET) for three weeks, the remaining group of animals being used as a control without treatment. In each group, the bacilli were inoculated through the caudal vein. Consequently, the Sakurai strain was found to be moderately virulent for mice. As to CER, a certain degree of therapeutic effect was observed for the infection with Sakurai strain, whereas CET revealed no therapeutic effect.<BR>(3) Inbred mice of DK1 strain were divi ded into two groups, and one was inoculated with Sakurai strain, the other with Kurono strain. Furthermore, each group was divided into four subgroups, and the first group received 250-1, 000 mg/kg of CER, the second group 200mg/kg of ethambutol (EB), the third group the same dose of CER and EB, and the last group served as a control. The drugs were daily administered for five weeks. The result showed that CER was effective for the group with Sakurai strain but not for the group with Kurono strain.<BR>(4) Histological study on the kidney of DK1 inbred mice was also performed to study the therapeutic effect and toxicity of CER. Daily doses of 500 mg, 1, 000 mg and 1, 500 mg/kg of CER were given subcutaneously to mice divided into three groups. The histological change was similar to that reported by Atkinson et al., which showed a reversible necrotic degeneration of epithelial cells of proximal tubules following one injection of CER intravenously.
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