抗結核剤耐性結核菌の走査電子顕微鏡的観察
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Morphological examinations of drug resistant colonies of mycobacterium tuberculosis were performed with the scanning electron microscope (SEM).<BR>Three strains (246, 55 & 69) of <I>M. tuberculosis</I> isolated from three patients were subjected to the study. The drug resistant colonies of these strains of <I>M. tuberculosis</I>, in vitro, were obtained either 1) through a series of subcultures on a gradually increased concentration of drugs, or 2) treatment with N-methyl-N'-nitro-N-nitrosoguanidine (NG). By thesemethodss, colonies grown on egg-medium (Ogawa) monoresistant to either of Streptomycin (1, 000μg/m<I>l</I>), Kanamycin (1, 000μg/m<I>l</I>), Para-aminosalicylic acid (100μg/m<I>l</I>) or Isoniazid (50μg/m<I>l</I>) were obtained, and they were abbreviated as SM1000-R, KM1000-R, PASloo-RR and INHso-RR. These single-drug resistant colonies were all rough in appearance on the egg-medium. Three-drug-resistant colonies, (SM-PAS-INH) lo-RR, were obtained by the same method mentioned-above.<BR>The surface of these drug resistant colonies of tubercule bacilli were observed with the SEM and it differed from the colonies of each parent sensitive strain in morphological features. The surface of the drug resistant colonies were more sticky than their parent strains; the filaments and granules were more prevalent in the drug resistant colonies. Occasionally, these drug resistant colonies were trapped with a net-like substance over the surface of the colonies. The morphologi cal changes related to the kind of drugs, i. e., KMiooo-RR colonies showed most marked changes, next PASloo-RR and INHso-RR, and least SMiooo-RR colonies. A slight difference was found between monodrug resistant strains obtained by methods No.1 and 2. The surface of the (SM PAS-INH) lo-RR colonies obtained by method No.1 showed some changes from the parent colo nies, while those obtained by method No.2 showed marked variation.<BR>It seems that these morphological changes of in vitro produced drug-resistant <I>M. tuberculosis</I> related to the emergence of drug resistance.
- 一般社団法人 日本結核病学会の論文
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