試験管内で誘導した薬剤耐性結核菌のPHファ-ジ感受性,およびSM耐性菌より得られた溶原株について

概要

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The phage PH susceptive and drug-sensitive M. tuberculosis isolated from patients were cultured on egg-medium containing the following drugs; SM, PAS, INH and RFP. Mono-drug resistant strains and tri-drug resistant strains of mycobacteria were obtained. A relationship between drug-resistance and a change in the phage susceptibility was indicated. The grade of diminution of the phage PH susceptibility in the mono-drug resistant mycobacteria-SM-1, 000 μg/ml, PAS-100 μg/ml, INH-50 μg/ml and RFP-100 μg/ml was 1/1, 1/10 or 1/100, respectively, as compared with those of the parent cultures in the former three drugs. The phage PH susceptibility of the RFP-resistant strain, however, had diminished more markedly than the other three agents (SM, PAS & INH), viz. 1/1, 000 compared with its parent strain. The tri-drug resistant mycobacteria, 3R-10 (SM 10 μg, PAS 10 μg, INH 10 μg/ml) did not lyse with the phage PH suspension containing 106/ml PFU. The phage PH non-susceptible lysogenic colonies appeared in the lysis-area by the phage spotting method on the drug-resistant cultures. The lysogenic mutant produced from the SM-resistant mycobacterium showed resistance not only to SM but also to other anti-tuberculous drugs such as PAS, INH, SF, PZA, KM, VM, EM, CPM, RFP, and CS.
一般社団法人日本結核病学会の論文