ツベルクリンアレルギ-に関する研究-2-受身伝達因子(TF)

概要

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There have been numerous reports on the transfer factor (TF) of delayed-type hypersensitivity, but there are still unsolved problems about its mechanisms. The experiments reported herein wereperformed in order to clarify the transfer mechanisms of tuberculin-type hypersensitivity (TTH). Usedas a TF was the non-dialyzable fraction of the extract obtained from spleen cells of guinea pigs vaccinated and challenged (VC group) with heat-killed BCG. 1. A fraction which showed TTH-transfer activity was isolated from the spleen extract of the VC group by column chromatography using a Sephadex G-75. It's molecular weight was 15, 000- 67, 000. 2. The low molecular fraction of TF did not contain H37Rv antigen or an antibody to H37Rv. 3. Recipient animals intravenously injected with the TF showed a positive skin reaction to PPDon the 3rd day, but the reaction was negative one week after the injection. 4. TTH was transferred to normal recipients with thymocytes or spleen cells from normal animalsincubated with TF in vitro for 24 hours, but not with the lymphnode cells of those animals. It was demonstrated that TTH could be transferred to normal recipients by thymocytes orspleen cells treated with TF in vitro. This results suggests that normal T cells are sensitized throughthe same process in recipient animals injected with TF.
一般社団法人日本結核病学会の論文