STC[2,3-diphenyl-5-thienyl-(2)-tetrazoliumchloride]の応用による結核菌発育の早期判定に関する研究 : 第2報STCの分離培養および直接謝性検査への応用に関する研究

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The usefulness of the application of STC—a new tetrazolium salt— for the earlier detection of bacillary growth in the mycobacterial examination using the stock strains and the isolated strains was presented in the report 1. This paper presents the results of the ap p lication of STC for the isolation culture and the direct resistance test. First, the influence of 1% NaOH treatment on the application of STC was examined. 10-3 and 10-5mg of H37Rv and Kurono strain treated with 1% NaOH were inoculated on 1% Ogawas media.0.1 Jul of 0.5% aqueous STC solution was added immediately, 1, 7 an d 14 days after the inoculation, and bacillary growth was examined at 10, 14, 21, 28 and 42 days after the inoculation. The results were shown in table 1. In the case of 1 NaOH treatment, the immediate addition of STC solution was also favourable for the earlier detection of bacillary growth, similar to the results presented in the report 1. The inhibitory effect of STC was not seen even in the case of immediate addition of STC solution. The addition of STC solution was applied for the direct resistance test in which the sputa treated with 1% NaOH were inoculated on 1% Ogawas media containing drugs. The results were shown in table 2. In the last case discharging atypical mycobacteria, the bacillary growth was seen earlier on the STC added media than the control media.
日本結核病学会の論文