EXPERIMENTAL STUDIES ON EFFECT OF COMBINED USE OF 1314 TH AND INH TO INH-RESISTANT TUBERCLE BACILLI:1. Combination effect in liquid medium and in vertical diffusion method

概要

論文の詳細を見る
We have repeatedly reported that in our clinical experience combined use of 1314Th and INH for patients having INH-resistant tubercle bacilli is more effective than the use of 1314Th alone. In the present study, experiments were conducted in order to confirm the combined effect of these drugs in vitro to INH-resitant tubercle bacilli. 1) By using Dubos' liquid medium, a hundred combinations of 1314Th and INH were prepared, each containing different concentrations of these drugs. In these media, H Rv strain resistant to 50γ/ml/ of INH was cultured for two weeks. Consequently, when 2. 5γ/ml and 1γ/ml/ of 1314Th were contained in the medium, the favourable effect of the addition of INH was observed (Table 1). 2) A similar effect was observed when Kirchner's medium was employed in place of Dubos' medium (Table 2). 3) Combination effect of 1314Th and INH was examined by using Ogawa's vertical diffusion method. When INH was used in combination with 1314Th in such high concentrations as 25γ/ml/ and 50γ/ml, the resulting inhibitory zones were much higher than those observed when 1314 Th was used alone (Fig. 1, Table 3).
一般社団法人日本結核病学会の論文