結核菌チール・ニールゼン染色法と浜崎ケトエノル顆粒染色法との関係
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概要
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Hamazaki ketoenol granule staining (KEG) is different from Ziebl-Neelsen staining on the point to iodide after chrome fixing. Comparisons were made between the said two staining methods after the fixing of Carnoy, formalin and Hamazaki chronic. The findings of those two methods were identical and especially when applied after Hamazaki chrome fixing, they were found to stain tubercle bacilli very strongly. As it was insisted by Hamazaki and confirmed by our follow-ups, the entity of KEG is either desoxyribose nucleotides or lipoid. Thus, the sections were fixed by digestion with alkaline or acid nucleo-ease and lipase or by Baryta water treatment in order to destroy the stainable substance.
- 日本結核病学会の論文