:Differentiation of BCG from Other Strains of Mycobacterium bovis

概要

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Acid-fast organisms isolated from tuberculous lymphadenitis-leison of two childrenvaccinated with BCG were identified as BCG. The following characteristics of the BCGstrain were considered as useful for differentiating BCG from other strains of M. bovis: 1) weakly positive reaction of the niacin test; 2) weakly positive reaction of the nitratereduction; 3) growth on a synthetic medium containing glucose as the sole source ofcarbon in the presence of ammoniacal nitrogen; 4) growth on a synthetic mediumcontaining nitrate as the sole source of nitrogen in the presence of glycerol-carbon; 5) growth on a modifed Sauton agar medium (glutamate was substituted for asparagine); 6) considerable growth on the Sauton agar medium containing 0.1% picric acid (pH7.0); 7) growth on Ogawa egg medium containing thiophene-2-carboxylic acid hydrazide, 1μg/ml 8) formation of rough eueonic colonies on Ogawa egg medium. In addition, the following two may be shown: (a) growth on Ogawa egg mediumcontaining sodium salicylate, 0.5mg/ml; (b) growth on Ogawa egg medium containing NH2OH. HCl, 0.125mg/ml. Other M. bovis strains do not show the above characteristics.
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