抗酸菌における薬剤耐性

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Viomycinresistant mutants were isolated from Mycobacterium smegniatig. Ribosomes were isolated and tested for drug resistance in subcellular systems containing poly (U) as messenger RNA. Resistance to viomycin in these strains was due to altered ribosomes. Further analysis showed that viomycin resistance was due to either altered 50 S or altered 30S subunits depending on the resistant strain (21). Viomycinresistant mutants with high level showed coresistance to streptomycin and kanamycin even though they were selected by viomycin only. On the contrary, streptomycin resistance with high level, selected by streptomycin only, did not show coresistance to viomycin (20). Viomycin reduced the amounts of dihydrostreptomycin to ribosomes of M. smegrnatis and Escherichia coli, although they have different modes of action. The dihydrostreptomycin binding to ribosomes could be exchanged with streptomycin or dihydrostreptomycin, but not with unrelated antibiotics, kanamycin, neomycin, spectinomycin, capreomycin, tuberactinomycin-N, chloramphenicol and erythromycin. It was suggested that there is a significant interaction between the binding sites of viomycin and streptomycin on ribosomes (26). From these evidence, biochemical basis of oneway cross resistance between streptomycin and viomycin was discussed.
日本結核病学会の論文