グラム陰性桿菌に分布する共通たんぱく抗原について

概要

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Distribution of cross-reacting protein antigen (CRPA) among 11 bacterial species was examined by immunoelectrophoresis. CRPA was detected in the sonicates of Shigella sonnei, Proteus mirabilis, Salmonella enteritidis, Klebsiella pneumoniae, Serratia marcescens, Escherichia coli, Yersinia enterocolitica, Pseudomonas aeruginosa and Vibrio cholerae but not in those of Neisseria gonorrhoeae or Staphylococcus aureus. A major protein with a molecular weight of 60 kilodaltons was found to be shared by the above nine species by SDS-PAGE and immunoblotting. CRPAs of V. cholerae and V. enterocolitica were partially purified by a combination of starch gel electrophoresis and gel filtration. The molecular weights of both CRPAs were estimated at about 500 kilodaltons by gel filtration. The 60 kilodalton protein was found also in both CRPAs by SDS-PAGE and immunoblotting, therefore, it is a major antigenic component of CRPAs of the above nine gram-negative rods. On the other hand, the major protein was not found in the outer membrane preparation obtained from V. cholerae. The present study suggests that CRPA is distinct from such previously reported common antigens as the outer membrane protein of V. cholerae and Kunin's antigen of enteric bacteria in the immunological and physico-chemical properties.
日本細菌学会の論文