極限条件で培養した中等度好塩菌の膜脂質組成とリポソ-ムの性状

概要

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Pseudomonas halosaccharolytica was grown under two extreme growth conditions of low temperature-low NaCl concentration (20C-0.5M NaCl) and high temperature-high NaCl concentration (40C-3.5M NaCl). High temperature-high NaCl-grown cells contained large amounts of saturated and cyclopropanoic fatty acids and acidic phospholipids, whereas low temperature-low NaCl-grown cells had decreased concentrations of these acidic phospholipids and fatty acids with increased concentrations of the corresponding unsaturated fatty acids. 14C tracer experiments with the resting cells showed that the high NaCl concentration inhibited the synthesis of phosphatidylethanolamine, but not that of phosphatidylglycerol. Liposomes of lipids extracted from the cells grown at 40C in 3.5M NaCl showed an increased the activation energy for altering the NaCl concentration of liposome buffer from 0.5M to 3.5M, whereas those of extractable lipids of the cells grown at 20C in 0.5M NaCl did not show such a change.
日本細菌学会の論文