実験的チフス症の研究-8f-

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The mouse super-immunized with live vaccine of Salmonella enteritidis acquired high antilethal resistance and survived intravenous injection with 1, 000 MLD of a fully virulent strain 116-54 of the same organism. In vitro studies demonstrated that the mononuclear phagocytes obtained from the abdominal cavity, liver or subcutaneous tissue of mouse super-immunized with live vaccine of S. enteritidisinhibited the intracellular multiplication of the same organism regardless of the presence of antibody in the cell culture medium, wherea3 the cells of mouse immunized with dead vaccine did not. This paper deals with the in vitro transfer of cellular immunity of mouse super-immunized with live vaccine of S. enteritidis to the mononuclear phagocytes obtained from normal mice. The supernatant fluid of monocyte culture (SFC) was obtained 24 hours after phagocytosis of heatkilled becteria from the cell culture of normal monocytes or the monocytes of immunized mouse. The SFC thus obtained was filtered through Millipore filter No.HA. When the normal monocytes were incubated for 3 days in cell culture medium containing 50% of SFC obtained from the monocytes of mouse immunized with live vaccine, the cells acquired the ability to inhibit the intracellular multiplication of virulent strain 116-54 and resisted the cellular degeneration caused by intracellular existence. of bacteria. But the normal monocytes treated with FSC obtained from normal monocytes or from the monocytes of mouse immunized with dead vaccine were unable to inhibit the intracellular multiplication of bacteria, and the cells were destroyed. The mechanism of antibacterial activity conferred by SFC of the mouse monocytes immunized with live vaccine of S. enteritidis is under investigation and will be described elswhere.
日本細菌学会の論文

実験的チフス症の研究-8f-

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