Electrophoretic study on the DNA-breaking actions of ascorbic acid and triose reductone in the presence of Cu2+.

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概要

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• The mode of DNA-breaking actions of ascorbic acid (AsA) and triose reductone (TR) in the presence of Cu2+ was studied by use of 0.8% agarose slab gel electrophoretic analysis. The DNA-breaking actions of the mixture of AsA or TR and Cue + were inhibited by N2 gas, hydroxyl radical scavengers and catalase, indicating that the oxidation of AsA or TR by Cu2+ and the hydrogen peroxide and hydroxyl radicals resulting from the oxidation are essential for the fragmentation of DNA by the mixture of AsA or TR and Cu2+. However, the DNA-breaking activity of these reductones varied with pH, while their oxidation rates were proportional to the increase in pH. The marked fragmentation of DNA occurred at pH 4, 7 or 8. The breakages of DNA by AsA or TR in the presence of Cu2+ were concluded not to be associated with the oxidation rates of reductones.

著者

• 西山 和夫

  Institute of Food Chemistry, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University

• 篠原 和毅

  Institute of Food Chemistry, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University

• 野中 美智子

  Institute of Food Chemistry, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University

• 村上 浩紀

  Institute of Food Chemistry, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University

• 大村 浩久

  Institute of Food Chemistry, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University

• 宗正 康

  Institute of Food Chemistry, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University

関連論文

• Function of Cu2+ on the DNA-breaking actions of ascorbic acid and triose reductone.
• Electrophoretic study on the DNA-breaking actions of ascorbic acid and triose reductone in the presence of Cu2+.
• .LAMBDA.DNA-fragmenting actions of ascorbic acid and triose reductone in the presence of Cu2+.
• Mutagenic action of triose reductone and ascorbic acid on Salmonella typhimurium AT 100 strain.

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