Ability of Endogenous Folate from Soy Protein Isolate to Maintain Plasma Homocysteine and Hepatic DNA Methylation during Methyl Group Depletion in Rats

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概要

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• We investigated different means of achieving methyl de pletion by feeding weanling rats modified AIN diets depleted of folate (FD), folate + choline (FCD), and folate + choline + methionine (FCMD), and examined the consequent effects on folate status, homocysteine levels, and methylation status. Control rats were fed a 12% protein diet consisting of either casein or soy protein isolate (SPI) and containing 2mg/kg folate, 0.2% choline, and 0.4% methionine. After the rats had been on the diets for 4 and 8 weeks, whole blood folate concentration was measured and found to be significantly depleted in the folate deficient treatments com pared with controls at 4 weeks (p <0.001), this reduction being significantly greater (p<0.03) in casein-fed rats (60%) than in SPI-fed rats (32%). The omission of choline and methionine from the diet had no further influence on whole blood folate. A significant inverse correlation was observed in the casein-fed rats after 8 weeks between mean plasma homocysteine concentration and decreasing methyl content of the diet (r2=0.978, p<0.002), an effect not seen in the corresponding SPI-fed rats. Hypo methylation of hepatic DNA evidenced by a reduction in 5-methylcytosine content was present in the casein rats fed FCD and FCMD relative to control (p<0.05). No hepatic DNA methylation changes were observed in the SPI-fed rats. The results obtained in the present work demonstrate that a soy-based diet can compensate against methyl group depletion by maintaining plasma homocysteine levels and an adequate level of DNA methylation, a result we attribute to endogenous folate content.

• 財団法人 学会誌刊行センターの論文

著者

• LEU Richard

  University of Adelaide, Department of Medicine, Adelaide

• MCINTOSHI Graeme

  CSIRO Division of Human Nutrition, Adelaide

• MCINTOSH Graeme

  CSIRO Division of Human Nutrition

• Le Leu

  University of Adelaide, Department of Medicine, Adelaide

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