Change in Blood Levels of Vitamin B-6 Derivatives in Pregnant and Lactating Rats.

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By using an HPLC method which we have developed, concentration changes of vitamin B-6 derivatives in blood of pregnant and lactating rats were studied. PLP and PL were the main derivatives in plasma and erythrocytes, and occasionally, PMP was found in the plasma of rats fed a normal solid diet which contained 8.3mg PN⋅HCl/kg diet. Upon pregnancy, the plasma PLP concentration decreased significantly (p<0.01), whereas plasma PL tended to increase (p<0.05). PLP con-centration in erythrocytes tended to increase upon pregnancy. These results suggest that metabolism or utilization of vitamin B-6 is altered upon pregnancy and that plasma PLP concentrations alone may not be a good indicator of nutritional status. Further confirmation was needed for the reliable determination of PMP because a large peak found at 7 min or so from erythrocyte samples under our analytical conditions was not converted to PIC-P via PLP, after the sample was treated with glyoxylic acid and then with KCN.