Correlation between Plasma α-Cysteine Proteinase Inhibitor Level and Pyruvate Kinase Activity in Vitamin E-Deficient Rats

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Weaning rats were divided into two groups, one group being fed a vitamin E-deficient diet, and the other an α-tocopherol-containing (50mg/kg) control diet. Rats were killed at 1, 2, 3 and 4 months of feeding. The following results were obtained. 1. Both plasma and liver α-tocopherol levels decreased greatly by feeding vitamin E-deficient diet for 1 month, and thereafter decreased gradually by continued feeding. 2. Somehow different results were obtained concerning liver perox-idation value by the method of analysis. In the case of chemilumines-cence, the value increased by vitamin E deficiency during the first 2 months, but thereafter, the value was almost unchanged. On the contra-ry, in the case of TBA-RS, the value increased gradually throughout the entire 4 months of feeding period. 3. Both plasma α-CPI level and pyruvate kinase activity increased by vitamin E deficiency, showing similar pattern of change with feeding period. Especially, marked increase of these values was observed in vitamin E-deficient rats fed for longer than 2 months, and differences from control groups were highly significant (in both cases, at 2 months, p<0.005, and at 3 and 4 months, p<0.001). And, in vitamin E-deficient group, including all the rats fed on test diet for 1 to 4 months, correlation between both values was very high, and was highly significant (r=0.9060, p<0.001).