Effects of vitamin E on toxicity by minute amounts of paraquat fed continuously to rats.

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The effects of vitamin E on toxicity by minute amounts of paraquat fed continuously for some period to rats were investigated. Two experiments were carried out as experiments 1 and 2. In both experiments, weaning rats were divided at first into two groups; one group was given a vitamin E-deficient diet, and the other a vitamin E-supplemented control diet (50mg α-tocopherol/kg of diet). They were fed on these diets for 40 days. After that, in both experiments, the rats that had been fed the vitamin E-deficient diet were further divided into two groups, which were either given a paraquat-added diet (+PQ-E) or continuously fed the same vitamin E-deficient diet (-E). The amount of paraquat added was 250 mg of methyl viologen per kg of diet. After the addition of paraquat, these two groups were pair-fed. In experiment 1, paraquat was given to all the rats fed the vitamin E-supplemented control diet (+PQ+E). In experiment 2, rats fed the control diet were divided into paraquat-added (+PQ+E) and non-paraquat-added (+ E) groups, similar to those of vitamin E-deficient rats. These two groups were also pair-fed thereafter. In both experiments, about 35 days after paraquat addition, they were sacrificed. Plasma and liver α-tocopherol contents were measured by HPLC, and liver peroxidation value was measured by chemiluminescence and the TBA method. And, as parameters of vitamin E deficiency, plasma pyruvate kinase and GOT activities and a-cysteine proteinase inhibitor (α-CPI) level were measured. When the analyzed values were compared between paraquat-added and the corresponding not-added control groups (+PQ-E vs. -E, +PQ+E vs. +E), the following results were obtained. In experiment 1, the values of plasma and liver α-tocopherol levels were significantly lower in the +PQ-E group than those of the -E group; however, liver peroxidation values and values of the three parameters of vitamin E deficiency were not different significantly. In experiment 2, the value of liver α-tocopherol level was significantly lower in the +PQ+E group than that of the +E group. As to liver peroxidation values, in the case of TBA reactive substance (TBA-RS), the value of the +PQ-E group was significantly higher than that of the -E group, and in the case of chemiluminescence, the value of the +PQ+E group was significantly higher than that of the +E group. As to the three parameters of vitamin E deficiency, the values of all three parameters were significantly higher in the +PQ-E group than those of the -E group, and the values of pyruvate kinase activity and α-CPI level were significantly higher in the +PQ+E group than those of the +E group. But, when ratios of weights of lung, kidney and liver to body weight were compared, these values increased significantly in both experiments by paraquat addition irrespective of the presence of vitamin E. Moreover, in experiment 2, occurrence of gross pathological signs were noticed in the paraquat-fed two groups.
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Effects of vitamin E on toxicity by minute amounts of paraquat fed continuously to rats.

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