Studies on bioassay and its application of an immunoregulatory and inflammatory lymphokine, interleukin 1 (IL-1).

概要

論文の詳細を見る
An improved assay method for interleukin 1 (IL-1) using murine (C3H/He) thymocytes has been developed.First, the responder cells to IL-1 among the thymocytes were characterized using the fractionated cells by peanut agglutinin (PNA), PNA-agglutinable (PNA+) and PNA-nonagglutinable (PNA-).PNA-cells, which were of Thyl+TL-TdT-matured medullary type, demonstrated substantial levels of proliferation after stimulation by human recombinant IL-1β(rIL-1β) in the absence of mitogens.Cells with a low density, which were isolated by Percoll-discontinuous density gradient and enriched of PNA-cells, also demonstrated a mitogenic response to rIL-1β in the absence of any other stimulants.This mitogenic response of PNA-thymocytes to rIL-1β was completely abrogated by removal of adherent cells from the PNA-cells or addition of monoclonal antibody against haplotype specific Ia antigen (Iak) in the cells, suggesting that the IL-1-induced proliferation of PNA-thymocytes may be dependent upon Ia antigens on the adherent cells.This IL-1 assay method using Iadependent response of PNA-thymocytes to IL-1 was found to be much more reproducible and reliable than the conventional concanavalin A co-stimulator assay method.Using the method, IL-1 activities either in the culture fluids of macrophages/granulocytes stimulated by inducers or those inside the cells could be easily determined.This PNA-thymocyte assay could also effectively be used for the suppressive effect of anti-inflammatory or anti-rheumatic drugs on macrophage IL-1 production.Thus, the method should be valuable for experimental and clinical studies of IL-1 production in healthy or diseased animals and people.
社団法人日本口腔外科学会の論文

Studies on bioassay and its application of an immunoregulatory and inflammatory lymphokine, interleukin 1 (IL-1).

スポンサーリンク

概要

著者

関連論文

スポンサーリンク