Evaluation of Lamination in Remineralized Enamel and Root Surfaces by Digital Image Processing.
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An <I>in vitro</I> pH-cycling experiment was performed to evaluate the changes in the mineral content in remineralized enamel and root surfaces. Tooth slabs were cut from intact human enamel and root tissue with cementum and were subjected to alternating demineralization (Dem) and remineralization (Rem) as follows: (1) enamel: A single cycle of 24-hr Dem (pH 4.0, 37°C) -10-day Rem (pH 7.0, 37°C) -24-hr Dem; (2) root: Four cycles of 2-hr Dem (pH 6.0, 20°C) -46-hr Rem (pH 7.0, 37°C) plus 2 cycles of 12-hr Dem (pH 5.0, 37°C) -36-hr Rem (pH 7.0, 37°C). Between the Dem and Rem cycles, both enamel and root slabs were immersed in 250ppm F solution for 1min. The slabs were sectioned at approximately 100μm and microradiographed. The mineral content in the tooth surfaces was assessed by digital image processing.<BR>The results were as follows:<BR>(1) The typical lamination resulting from pH-cycling was observed both in enamel and root surfaces.<BR>(2) The mineral distribution was different between enamel and root surfaces, e. g., the width of the second Dem layer of enamel was much narrower than that of the first Dem layer, whereas both of the Dem layers in root surfaces showed similar width.<BR>(3) The mineral volume of the middle part of the Dem layer was lower than that of the edge, but this mineral distribution was reversed in the Rem layer, and each layer showed a "sandwich" structure.<BR>(4) Digital image processing of microradiographs was useful for evaluating the mineral volume in the remineralized tooth samples. Particularly the use of a pseudo-color display for monochrome images was useful for evaluating mineral distribution visually.
- 有限責任中間法人 日本口腔衛生学会の論文
有限責任中間法人 日本口腔衛生学会 | 論文
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