Prevention of experimental periodontal disease by immunization with cell surface antigen extracted from Actinomyces viscosus.
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概要
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The purpose of this study was to investigate the effect of immunization with the cell surface antigen extracted from <I>Actinomyces viscosus</I> on periodontal disease of hamsters, which was induced by inoculation with <I>A. viscosus</I> K-TL <SUP>+</SUP>.<BR>Cell surface substances were released from <I>A. viscosus</I> K-TL<SUP>+</SUP> cells by stirring them in 0.05M Tris-HCl buffer, pH 7.4, containing 1M NaCl. These were purified by ammonium sulfate precipitations, gel filtration and ion-exchange chromatography, and fibril-rich substance was obtained. This prepaation was used as an immunogen.<BR>Golden hamsters were divided into three groups, immunized, shamimmunized and diet-control groups, and they were immunized in their pouches at 4 and 5 weeks of age. At six weeks of age, they were infected with <I>A. viscosus</I> K-TL<SUP>+</SUP>, and the establishment of the strain was examined during the experiment. At the end of the experiment, 8 weeks after the last immunization, the hamsters were killed after sampling their saliva and sera, and the mandibulars were evaluated for their alveoral bone loss and root surface caries.<BR>In the immunized group, the mean recovery of <I>A. viscosus</I> K-TL<SUP>+</SUP> was less, and the occurrence of alveolar bone loss and root surface caries considerbly decreased comparing with the sham-immunized group. Both saliva and sera obtained from hamsters in the immunized group also showed more elevated levels of antibody against cell surface antigens than in the sham-immunized group.<BR>The results suggested that immunization with the cell surface antigen might be effective in prevening periodontal diseases induced by <I>A. viscosus</I>.
- 有限責任中間法人 日本口腔衛生学会の論文
有限責任中間法人 日本口腔衛生学会 | 論文
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