Binding of oral bacteria with the sialic acid-rich glycoprotein purified from human submandibular-sublingual saliva.

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Glycoproteins in saliva play an important role in dental plaque formation. This study deals with the binding of oral bacteria with the sialic acid-rich glycoprotein purified from human submandibular-sublingual saliva. Sialoglycoprotein was isolated and purified from human submandibular-sublingual saliva by fast protein liquid chromatography followed by high performance liquid chromatography (HPLC). The molecular weight of the purified sample, consisting of four subunits, was estimated to be 440K daltons by both the HPLC and SDS-disk electrophoresis. Both Streptococcus sanguis and Bacteroides gingivalis had high binding affinity to sialoglycoprotein. When the cell surfaces of both bacteria were treated with trypsin at 37°C for 90minutes, the decrease of radioactivity was less in Streptococcus sanguis than Bacteroides gingivalis. No radioactivity was detectable in the 2 pieces of bacteria after heating at 100°C for 10minutes, indicating the lost of binding ability.
有限責任中間法人日本口腔衛生学会の論文