ラット急性フッ素中毒における血清,唾液腺コリンエステラ-ゼ活性値の変動

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Fluoride has an inhibitory effect on cholinesterase (ChE) in vitro, so the effects of sodium fluoride administration on activities of ChE in serum, brain and salivary glands were studied in female rats. ChE activies in them were measured by the colorimetric method of Ellman et al. two percent NaF solution was administered orally to female Wister rats and the animals were sacrificed by heart puncture 1, 3, 24 and 48 hours after a single dose to remove the various organs and the blood.<BR>Fluoride analysis was performed for all the serum, brains, kidneys, and salivary glands and ChE activity in serum, brains and salivary glands. In fluoride administrated animals the fluoride concentration in the serum, kidney, and salivary glands was about one hundred times higher than control rats at one hour after a single dose of NaF.<BR>Peak inhibition of ChE occurred a few hours after administration of NaF per os. The ChE inhibition caused NaF elevated acetylcholine concetration, so that acute intoxication resulted in signs of parasympathetic stimulation such as salivation and nausea.
有限責任中間法人日本口腔衛生学会の論文