Effect of ammonium fluoride in human enamel and rat experimental caries.
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概要
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The purpose of this study was to evaluate the possibility of clinical use for fluoride topical application of dilute ammonium fluoride soluion, in which fluoride concentration was 1/3 times lower than that of APF solution.<BR>Experiments were performed <I>in vitro</I> and <I>in vivo</I> using a solution of phosphoric acid-acidified NH<SUB>4</SUB>F (3000ppm of F-, pH 4.4) and APF (9000ppm of F-, pH 3.4). In the experiments on intact enamel, fluoride uptake, acid solubility, and morphological changes in enamel blocks following topical treatment (for 60min. at 37°C) with fluoride solutions were studied by chemical analysis, scanning electron microscopy, and electron probe X-ray microanalysis. In experiments on rats, the cariostatic effect of ammonium fluoride solution was investigated. Eighteen-day-old male Sprague-Dawley rats were infected with <I>Streptococcus mutans</I> GS-5 for three days and fed a cariogenic diet (Diet 2000 No. 3) during the experimental period. Experimental groups were given topical application of each fluoride solution twice a week. Caries prevalence was examined using a standardized photograph (×50) of the rat jaw, and then caries scores (caries incidence and caries extent) were calculated from these data.<BR>The fluoride uptake of NH<SUB>4</SUB>F-treated enamel surface was a few times higher than that of APF-treated enamel. In the electron probe X-ray microanalysis, the same results were obtained. The acid solubility test proved the acquisition of acid resistance by the enamel. SEM images showed heavy deposits of crystalline material on NH<SUB>4</SUB>F-treated enamel surface, whereas the deposit was homogeneous in APF-treated enamel.<BR>Both caries incidence and caries extent were clearly reduced, and significantly lower values were found in the fluoride groups than in the control group. The fluoride groups had very few serious grade lesions, whereas the control group had many collapsible lesions.<BR>It is concluded that the topical application of dilute NH<SUB>4</SUB>F solution leads to uptake of fluoride into the enamel surface and proves the acquisition of acid resistance by enamel <I>in vitro</I>, and is effective in reducing caries incidence and inhibiting caries progression in rat experimental caries <I>in vivo</I>. A dilute NH<SUB>4</SUB>F solution can be expected to have the same effect as APF in topical application for caries prevention.
- 有限責任中間法人 日本口腔衛生学会の論文
有限責任中間法人 日本口腔衛生学会 | 論文
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