〔食用きのこの深部培養に関する研究-1-〕食用きのこの深部培養における炭素源と窒素源について

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Recently, the submerged culture of basidiomycetes has been arousing interest not only in the artificial cultivation of edible fungi but also in the microbial production of enzymes, antibiotics and carcinostatic substances. With a view to consolidate the technical basis of submerged culture of edible fungi, Lentinus edodes and Pleurotus ostreatus, a series of studies with the nutrients of culture medium have been undertaken. The dry weight of mycelium obtained from the media containing glucose as the carbon source was 10g/l for L. edodes and 15g/l for P. ostreatus respectively. The addition of mucous substances, sodium alginate and methyl cellulose, to the media proved to be effective to reduce the pellet size of P. ostreatus, but not to stimulate growth. Use of starch in place of glucose as the carbon source in the media almost trebled the yield of L. edodes. However, the subculture with this media tended to fluctuate in mycelium yield considerably. Such a problem of fluctuation in the subculture was found to be remarkably improved by incorporating glucose at the concentration of 2_??_3% to the starch media, but when used at 4_??_5% glucose exhibited the inhibitory effect for the growth of L. edodes. The mixture of zinc chloride and copper sulfate was effective to stimulate the growth of L. edodes when corn steep liquor (CSL) was used as the nitrogen source in the media. In conclusion, the maximum dry weight of mycelium obtained from the media containing starch, glucose and CSL reached 25g/l for L. edodes in 14 days and 27g/l for P. ostreatus in 6 days respectively.
社団法人日本農芸化学会の論文

〔食用きのこの深部培養に関する研究-1-〕食用きのこの深部培養における炭素源と窒素源について

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