〔食用きのこの深部培養に関する研究-2-〕食用きのこのデンプン高濃度培養について

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The growth-stimulating effect of culture nutrients and other chemicals in the high consistency starch media has been studied in the submerged culture of edible fungi, Lentinus edodes and Pleurotus ostreatus. The maximum dry weight of mycelium botained in this study was 45g/l, which was reached both with L. edodes and P. ostreatus in 11 days and 6 days respectively. The growth rate of P. ostreatus was 7g/l/day which was much greater than that of L. edodes. The optimum concentration of medium composition to ensure these results was 70_??_90g/l starch, 10g/l glucose and 15_??_20g/l corn steep liquor (CSL) for L. edodes, and 70_??_100g/l starch, 10g/l glucose and 20_??_25g/l CSL for P. ostreatus. The addition of potassium phosphate and zinc chloride as mineral completes the culture formulation in either case. Using sucrose in place of glucose in the starch-glucose-CSL (SGC) media, it exhibited the same growth-stimulating effect as glucose, and the effect was maintained even at the sugar concentrations as high as 7% which was known to show the growth-inhibiting effect for glucose. The addition of sucrose fatty acid ester, nonionic surfactant, to the SGC media was effective to stimulate the growth, but not to increase the ratio of filamentous mycelia. Substitution of yeast extract or peptone for CSL, and partial enzymatic decomposition of starch in the SGC media not favor the growth of L. edodes. The relation between the growth of L. edodes and the change of sugar concentration was also discussed.
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〔食用きのこの深部培養に関する研究-2-〕食用きのこのデンプン高濃度培養について

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