発生過程にある葉緑体のチオ-ル基と光環元活性

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Changes in thiol content of chloroplast during photoinduced development as well as the relationship between the thiol content and the photoreductive activity of chloroplast were investigated. Thiols of 14.8, μmoles on 100 μmoles chlorophyll basis were detected by DTNB in the isotonic solution (0.4M sucrose) and 30.5 in the hypotonic solution (0.13M sucrose), for the chloroplast from 72 hr illuminated radish cotyledons. The thiol content of the developing chloroplast on a protein basis was shown to be enhanced until 48 hr illumination and then to decline. The thiol content on a chlorophyll basis decreased with the illumination time even after the stationary state of chlorophyll content. The content in a matured leaf of the radish was as 1/20 as that of the chloroplast in an initial stage of the development. These results indicate that the underdeveloping chloroplast occurs in comparably reduced state and changes gradually to less reduced state with time. The thiol content of the chloroplast was diminished with some treatments which could depress the PS-II activity of the chloroplast. When the thiols were blocked with a sulfhydryl reagent such as pCMB or DTNB, the PS-II activity was also depressed, but the PS-I was not. On 15min illumination, the thiol content as well as the photoreductive activity of the chloroplast were enhanced but not in the presence of DCMU as an inhibitor of photosynthesis. Based upon the results as described above, the close relationship between the thiol content and the function of the developing chloroplast was discussed.
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発生過程にある葉緑体のチオ-ル基と光環元活性

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