デキストラナーゼ精製酵素の諸性質(Aspergillus ustus 237の生産するデキストラナーゼに関する研究-2-)

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概要

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• Dextranase which was produced by Aspergillus ustus 237 was purified to approximately 1000 folds by means of ammonium sulfate fractionation, DEAF-cellulose column. chromatography and Bio-Gel P-150 column chromatography. Purified enzyme was appeared to be homogenous in disc-electrophoretic analysis and its molecular weight was estimated to be approximately 35, 000 by gel-filtration on Bio-Gel P-150. Optimum pH and temperature for the enzyme reaction were pH 5.7 and 50°C, respectively. The enzyme was stable in the pH range of 5.5 to 8.5 and also at the temperatures less than 40°C, while more or less 50% of the enzyme activities were inactivated by heating to 50°C for 15 minutes at pH 7.0. However, it was found that the enzyme showed the remarked heat-tolerance in the presence of some cations, such as Cr3+ and Al3+. The dextranase activity was inhibited completely by Hg2+, Ag+ and permanganate and was scarcely by chelating agents reductants.

• 社団法人 日本農芸化学会の論文

著者

• 花田 香一

  明治製糖株式会社研究所

• 広瀬 明

  東日本製糖株式会社出向中

• 二宮 英二

  明治製糖株式会社研究所

関連論文

• 一有胞子細菌の生産する高粘性多糖類(第1報) : 生産物の確認
• 一有胞子細菌の生産する高粘性多糖類(第2報) : 物理的性質と構成糖比
• デキストラナーゼ精製酵素の諸性質(Aspergillus ustus 237の生産するデキストラナーゼに関する研究-2-)
• デキストラナーゼ生産菌の分離同定および培養条件(Aspergillus ustus 237の生産するデキストラナーゼに関する研究-1-)

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