B.cereusの胞子および栄養細胞に含まれるタンパク質のマクロレベルにおける比較(ノート)

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The N-terminal amino acid of S-100 (soluble fraction) and ribosomal proteins from spores of B. cereus were mainly Ala, Ser (Met), and Thr. The results were very similar to those obtained with corresponding samples from vegetative cells and with the results reported for proteins from E. coli. The average molecular weight of spore S-100 proteins (31, 000) was also about the same as that of vegetative cell proteins (25, 000). These facts presumably suggest that the proteins in spores are synthesized by the ordinary mechanisms of protein synthesis. Amino acid analyses revealed that spore S-100 proteins were more acidic and spore ribosomal proteins were perhaps rather more basic than the corresponding proteins from vegetative cells and from E. coli. During the course of amino acid analyses of spore S-100 proteins one unusual peak (X) was found at a position between peaks for methionine and isoleucine. The substance X, presumably amino acid, was detected exclusively in spore S-100 proteins and appears to be contained in proteins or other high molecular weight substances. It will be of interest to investigate the mode of binding of X to high molecular weight substances, and biosynthetic route by which it is incorporated into them.
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