ラムノース培地培養酵母の脂質からのジオールの検出

概要

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In order to study the biogenesis of diol-lipid, it was attempted to investigate the condition under which diol-lipids were produced more effectively in yeast. Among six species used, four species have been observed to produce free 1, 2-propanediol into the fermented broth, when they were cultured in the medium containing L-rhamnose. The other two species have been reported to produce and accumulate large quantities of lipids. These six species were first cultured in the medium containing glucose for 48 hr, followed by succeeding cultivation in the medium containing L-rhamnose for further 48 hr. Total lipid fraction which had been extracted from lyophilized cells was hydrolyzed by alcoholic alkaline solution, followed by analyzing the polyol fraction by thin-layer chromatography and gas-liquid chromatography. 1, 2-Propanediol was detected in the lipid hydrolyzates of five species. No diol was found in the lipid hydrolyzates cultured in the medium without L-rhamnose. These results suggest that 1, 2-propanediol in the lipid hydrolyzates was derived from L-rhamnose.
社団法人日本農芸化学会の論文