Thiamine要求性Corynebacterium属細菌のグルコン酸代謝に関する研究(第3報) : ピルビン酸の蓄積(2)

概要

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It was reported in the preceding paper that Corynebacterium sp. No. 208 required thiamine for growth and accumulated a large amount of pyruvate in the extracellular fluid when it was cultivated in the thiamine-deficient medium. The present investigation was undertaken to demonstrate the effect of thiamine on pyruvate accumulation with the suspensions of resting cells grown in the thiamine-deficient and thiamine-sufficient media. The thiamine-deficient cells which were able to produce pyruvate from gluconate showed poor utilizing-ability for pyruvate added to the suspension as a substrate, and the ability was increased by the addition of thiamine. Pyruvate was also oxidized at a decreased rate by the suspension of thiamine-deficient cells. Moreover, the thiamine-deficient cells demonstrated poor oxidative ability for acetate, lactate, malate, fumarate and α-ketoglutarate. Resting cells grown in the thiamine-deficient medium supplemented with iron utilized and oxidized pyruvate and other organic acids described above at a increased rate. The addition of arsenite gave a increase in the accumulation of pyruvate. It is possible to conclude from the present results that the accumulation of pyruvate by the thiamine-deficient cells is a consequence of the low level of pyruvate dehydrogenase in the cells.