バイラス病体のプロテアーゼに関する研究〔第1報〕家蚕膿病体のプロテアーゼ作用に及ぼす水素イオン濃度及び加温の影響-

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The multiplication of viruses depends upon the denaturation or polymerization of normal protoplasma proteins in living tissues. Therefore, in order to clarify the mechanism of virus formation the metabolism in the cells must be investigated. As it has been generally recognized that the virus molecules are no more than the polymerized nucleoproteins, I have begun to study the changes and properties of proteases in the virus-diseased tissues. At first, the investigation on the nature of proteolytic enzymes in polyhedral-diseased silkworms was performed. The silkworm is the most suitable animal for this purpose, because it grows very rapidly and chemical changes in its body are vigorous. Furtheremore, this insect is easily attacked with a virus disease. The polyhedral-diseased silkworms were treated with acetone and an enzyme praparation was prepared. The proteolytic activity of this preparation is somewhat low in acid side, most weak at neutral reaction and strong at higher pH. The action of the protease is very weak below 10°, strongest at 50° and over 70° the activity is losta in few minutes. At all times it was found that the protease activity of diseased body is considerably higher than the activity of healthy one.
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