純蛋白質定量に用いる蛋白質沈澱劑に就て食品及び飼料成分の分析法に關する研究第2報

概要

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We designed to study the relation among the results obtained by the BARNSTEINs method, trichloroacetic acid and perchloric acid method for the determination of true protein in foods and fodders. Our results indicate that (1) the BARNSTEINs reagent precipitates some non-pro-teinous nitrogen compounds in foods and fodders, especially in hay and rice bran, and so it is not, suitable for the purpose; (2) trichloroacetic acid method is the best of all the three by our results, and we should use the 5_??_10% CCl3COOH solutions and wash the precipitation by the same solution; and (3) perchloric acid method is not applicable generally in the protein determinations of foods and fodders.
社団法人日本農芸化学会の論文