微生物による酒石酸の分解について : (第3報) Pseudomonas incognita Strain 3 Lによる嫌気的酒石酸分解反応の中間体としての燐グリセリン酸及びオキザロ醋酸の検討

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概要

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• 1. From the results obtained in the previous C14O2 fixation experiment two schemes(Scheme 1 and 2) were proposed to explain C14 incorporations into the various substances. Following experimental results in this report exclude the possibility of Scheme 1 as the main path of the anaerobic decomposition of tartarate by this organism. (1) D-(-)-Phosphoglycerate was decomposed anaerobically and about 1 mole of CO2 was evolved per mole of phosphoglycerate consumed (Table 2), while 1/3-mole of CO2 should be, expected if this compound were metabolized according to Scheme 1 (See eq. (13) in Table 7). So CO2 fixation reaction according to Scheme 1, that is eq. (13), did not occur, under these conditions. From the thermodynamic point, of view the CO2 fixation reaction according to Scheme 1 may be performed more easily when phosphoglycerate rather than tartarate is the -substrate (Table 7). Therefore this experimental result makes Scheme 1 unlikely. (2) Similarly succinate formation by the CO2 fixation reaction was not observed in the anaerobic decomposition of pyruvate (Table 6). (3) NaF, in the final concentration of M/600, completely inhibited the decomposition of phosphoglycerate, whereas the decomposition of tartarate was not inhibited in the same concentration and no phosphoglycerate was accumulated even when the tartarate decomposition was partially inhibited by the higher concentration of NaF (Table 3). Therefore phosphogly-cerate was excluded as the intermediate. (4) Anaerobically DL-glycerate was not metabolized by this organism and not likely to be the intermediate (Table 1). 2. Both oxalacetate decarboxylation and tartarate decomposition were inhibited by dipyridyl (M/340) and malonate (M/40) almost to the same degree (40 to 60%) (Table 4). These results support strongly the possibility of occurence of Scheme 2. 3. Anaerobic decomposition of malic acid was studied quantitatively and was found to be metabolized according to equation (9) as expected from Scheme 2. 4. From these results it is very possible that the anaerobic decomposition of tartarate is performed according to Scheme 2 by this organism.

• 社団法人 日本農芸化学会の論文

著者

• 野村 真康

  東京大学農学部農芸化学科

関連論文

• 菌類による左旋性エチレンオキサイド-α, β-ヂカルボン酸の生産 : (第6報) C14O2存在下におけるMonilia formosaの醗酵
• 微生物による酒石酸の分解について : (第3報) Pseudomonas incognita Strain 3 Lによる嫌気的酒石酸分解反応の中間体としての燐グリセリン酸及びオキザロ醋酸の検討
• 放射性同位元素による微生物の物質代謝に關する研究 : 酵母及び絲状菌の燐酸代謝に及ぼす諸條件の検討

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