醗酵によるVitamin Cの製造-7-
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概要
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The conditions affecting the production of Ca-5-keto-gluconate from glucose by shaking culture of Acetobacter suboxydans, strain 621, were studied. The relation curve between pH value and the activity of the oxydation of Ca-gluconate to Ca-5-keto-gluconate in cell suspension showed an optimum at pH 4.0. Since the optimal pH for the accumlation of Ca-5-keto-gluconate was very strict, it was suggested that the amount of CaCO3 added should be controlled in order to affect the optimal conditions. It was shown that suitable amount of fish extract and CaCO3 were 0.5% and 2.45% respectively per 10% glucose (purity 98.5%) media. Yields of 90.2% of Ca-5-keto-gluconate to glucose have been obtained.
- 社団法人 日本農芸化学会の論文