微生物によるパントテン酸の生合成に関する研究 : (第2報) Pantoic acidおよびその前駆物質の定量について(その2)

概要

論文の詳細を見る
The estimation of pantoic acid (PA) and its precursors contained in the enzymatic reaction mixture was studied. The results were as follows: (1) α-Ketopantoic acid (KPA) was determined by means of the specific color reaction of 2, 4-dinitrophenylhydrazone of a-ketopantolactone (KPL-DNP), with ammonia water (cf. Part 2 No 1). The conversion of a-ketpoantolactone (KPL) to KPL-DNP was promoted by the addition of hydrochloric acid and especially ethanol. The condition of the quantitative formation of KPL-DNP and the standard curve for determination are shown in Table 2 and Figure 4, respec-tively. (2) β, β-Dimethylpyruvic acid (DMPA) was determined by measuring the red color due to the alkalinized 2, 4-dinitrophenylhydrazone of DMPA (DMPA-DNP). The standard curve is shown in Figure 9. The steric structure of DMPA-DNP was assigned to the syn configuration from the infrared absorption spectra data and the difficulty of isomerization to an anti isomer may be attributed to effects of steric hindrance caused by the terminal methyl groups of DMPA-DNP molecule. (3) The total amount of KPA and PA was determined by the color reaction of KPL and pantolactone (PL) using 2, 7-naphthalenediol reagent. The standard curves are shown in Figure 11. (4) RF values of pantothenic acid precursors and their related compounds are shown in Table 4.
社団法人日本農芸化学会の論文