カーネーションの組織培養に関する研究-2-培養カルスの細胞学的観察
スポンサーリンク
概要
- 論文の詳細を見る
The callus used in this study was obtained from the shoot apices culture and has been subcultured every thirty days for over two years on Murashige and Skoog′s agar medium.The results obtained are as follows:1. The rate of the callus growth was rapid till about thirty days after the subculture of the callus, then, it became gradually slow.2. In callus subcultured continuously for over two years, the formation of vessels was observed but the adventitious root was not formed.3. The callus consists of normal and abnormal cells. Abnormal cells were not normal in size, shape and number of nucleus, and callus growth mainly depends on division of normal cells.4. The chromosome number in meristematic parts of the callus was abnormal in most cases, but diploid (2n=30) was rarely observed and normal mitosis was observed in it.