Effects of oxygen free radicals on cell growth and productions of .ALPHA.-fetoprotein(AFP) and albumin(ALB) by human hepatoma cell line(HH2-6).

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Secreting and producing capacities of α-fetoprotein (AFP) and albumin (ALB) by human hepatoma cell line (HH2-6) exposed to oxygen free radicals generated by dihydroxyfumarate (DHF) were studied in vitro. It was found that cell number were declined in proportion to DHF concentrations for 48hrs culture and cell proliferations were inhibited by DHF in growth curve. The amount of AFP secreted per cell (secreting capacity) was decreased at high concentration of DHF (50μg/ml) for 48hrs culture, and remarkable elevation of AFP-secreting capacity for growth stage was inhibited even by DHF (10μg/ml). On the other hand, ALB-secreting capacity was not affected with DHF. Producing capacities of AFP and ALB were correlated with secreting capacities. Cu, Zn-superoxide dismutase (Cu, Zn-SOD) interfered these reactions. These results suggest that oxygen free radicals inhibit cell proliferations and suppress AFP-secreting and producing capacities of HH2-6 cells selectively.
財団法人日本消化器病学会の論文