Role of neutrophils-derived oxygen radicals for the formation of rat gastric mucosal injury by ischemia-reinfusion.

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概要

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• The implication of neutrophils-derived oxygen radicals in the formation of ischemia-reinfusion (I-R) injury in the rat stomach was evaluated.Male SD rats were subjected to 20min hypotension and 20min reinfusion of shed blood as we described previously (Gastroenterology 88: 1162, 1985). The area of gross gastric lesions was measured and scores of histologic damage assessed by our criteria as reported previously (Gastroenterology 94: 1135, 1988). Luminol-dependent chemiluminescence (CL) was assessed in blood samples withdrawn from the portal vein and abdominal aorta immediately before killing rats. In a study prior to the I-R experiment, 3ml of anti-neutrophils monoclonal antibody (ANA) given i.p. was confirmed to significantly reduce the number of neutrophils in blood from the carotid artery with the reduction rate being constant by 6 to 18hr after. On the basis of this results, rats were administered ANA i.p. at the same dose 12hr before I-R. Controls received normal rat serum.The area of gross gastric lesions in the corpus (69vs. 191mm2), but not in the antrum (13vs. 20 mm3), was significantly reduced in the ANA group compared to that in control. The scores of histologic damage was significantly smaller in both the corpus (1.2vs. 2.0) and antrum (0.8vs. 1.5) in the ANA group than in the control. ANA significantly reduced the number of neutrophils and the CL levels in blood from both the portal vein (number, 107vs. 915; CL, 26vs. 235 RLU) and abdominal aorta (number, 52vs. 368; CL, 24vs. 268 RLU) in the ANA groups as compared to controls. There was no significant difference in the CL activity of each neutrophil from both blood samples between the ANA and control groups.These results suggest that oxygen radicals derived from neutrophils may be implicated in the formation of gastric injury induced by ischemia-reinfusion in the rat.

• 財団法人 日本消化器病学会の論文

著者

• 仙道 富士郎

  山形大学医学部

• 岩井 彰

  名古屋市立大学医学部第一内科学教室

• 安江 直二

  山形大学医学部寄生虫学教室

• 伊藤 誠

  山形大学医学部寄生虫学教室

• 横山 善文

  山形大学医学部寄生虫学教室

• 川合 孝

  山形大学医学部寄生虫学教室

• 松葉 周三

  山形大学医学部寄生虫学教室

• 岡山 直司

  山形大学医学部寄生虫学教室

• 石川 進

  山形大学医学部寄生虫学教室

• 加藤 直也

  山形大学医学部寄生虫学教室

• 武内 俊彦

  山形大学医学部寄生虫学教室

• 岩井 彰

  名古屋市立大学医学部第1内科

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• Role of neutrophils-derived oxygen radicals for the formation of rat gastric mucosal injury by ischemia-reinfusion.

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