A new simplified colorimetric assay of colipase in duodenal juice using BALB as substrate and its clinical application.

概要

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The method for measurement of the colipase concentration in duodenal fluid using artificial substrate BALB was established. The pattern of colipase secretion in C-S test and the relation between the pancreatic secretion of enzymes and the output of colipase were investigated. The measurement using BALB permitted easy and simultaneous analysis of a large number of samples. The results well correlated with the titrimetric method. The colipase secretion in C-S test remarkably increased by stimulation with caerulein but manifested no significant reaction to stimulation with secretin. On examining the secretion of pancreatic enzymes during C-S test in patients with mild to moderate pancreatic dysfunction, the outputs of colipase, lipase and amylase amounted to 72.6%, 72.9% and 94.7% of those in normal controls, respectively. The measurement of the colipase concentration in duodenal fluid in C-S test was found useful in the diagnosis of mild pancreatic dysfunction.
財団法人日本消化器病学会の論文