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The curreut investigation was designed to developed a radioimmunoassay method of pancreatic elastase in rats and to study the elastase levels.The presence of serum elastase inhibitors did not have an effect of the radioimmunoassay with the active sitespecific reagent Di-iso-propylfluorophosphate. The molecular size distribution of 125I-elastase or DFP- 125I-elastase after incubation with normal rat serum by Sephadex G-200 filtration suggested that 125I-elastase was bound to α2-macroglobulin and α1-antitrypsin, however DFP- 125I-elastase did not. Radioimmunoassayable elastase in serum was elastase-α1-antitrypsin complexes, following gel filtration of pooled serum with added exogenous elastase. There were no cross-react between human elastase I, procine elastase and bovine trypsin. A mean of 127ng/ml in normal rat serum have been determined and the minimal detectable amount was 4.7ng/ml.These results suggested that the radioimmunoassay for rat elastase which has been developed in this experiment is a specific and highly sensitive method for pancreatic elastase in rats.
財団法人日本消化器病学会の論文