RADIOIMMUNOASSAY FOR SUBSTANCE P AND PLASMA LEVELS OF SUBSTANCE P LIKE IMMUNOREACTIVITY IN THE PATIENTS WITH HEPATIC DISEASE AND HYPERTHYROIDISM

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The present paper reports a sensitive and specific radioimmunoassay for Substance P (S-P) by using synthetic S-P as standard and Nα-Tyrosyl-S-P as tracer. The double antibody phase reparation technique was employed to separate the labelled Nα-Tyrosyl-S-P from damaged component and unreacted iodine. No cross-reaction were observed in this system by various hormones, i.e., VIP, ACTH, Glucagon, Gastrin, Secretin, Motillin, Calcitonin and CCK-PZ. The minimum detection limit was 120 pg/ml. Using this system, human plasma samples from healthy volunteers and various patients were detected to present the S-P like immunoreactivity. In normal subjects plasma S-P levels ranged from 300 pg/ml to 650 pg/ml and the average was 445 pg/ml. High plasma S-P levels were found in the patients with hepatic diseases, hyperthyroidism, chronic renal failure and shizophrenia, but not in the patients with peptic ulcer and medullary carcinoma of the thyroid. Hyperthyroid patients showed high S-P levels with high T3 and T4 levels.In hepatic diseases S-P levels were significantly elevated by ductal enzyms, i.e., LAP, Al-P and γ-GTP, but not by hepatocellular enzyms, i.e., GOT and GPT. After ligation of common bile duct of the dogs plasma S-P levels elevated and remaind high. In addition experimental obstruction of bile duct changed plasma S-P levels. The mechanism of which remains to be studied.
財団法人日本消化器病学会の論文