オートアナライザーによる油脂及び油脂誘導体中の遊離脂肪酸の自動比色定量法

スポンサーリンク

概要

• 論文の詳細を見る

• An automated colorimetric method based on continuous flow analysis has been developed for determining free fatty acids in fats and oils and their derivatives.<BR>A 2-propanol solution of a sample was directly introduced into a Technicon Auto Analyzer. <I>o</I>-Nitrophenylhydrazine (ONPH) solution and <I>N</I>-ethyl-<I>N</I>- [3- (<I>N</I>, <I>N</I> methylamino) propyl] carbodi-imide (EDC) solution containing 5% pyridine were added to the sample solution, followed by heating at 60°C for 12min. The free fatty acids in the sample were converted the corresponding <I>o</I>-nitrophenylhydrazides. The solution was mixed with 1% KOH solution to decrease the absorbance of the reagent blank, followed by measurement at 550nm. The method provides a common molar response to a series of fatty acid from caprylic acid to oleic acid. The sample capacity of themethod is 2040 samples/h. Its reproducibility is within 1% relative and recovery, from of 97100%. It was successfully used for determining lipase activity and quantitating free fatty acids in various fatty acid derivatives.

• 社団法人 日本油化学会の論文

著者

• 高野 敏

  花王栃研

• 徳田 一

  花王 (株) 栃木研究所

• 高野 敏

  花王 (株) 栃木研究所

関連論文

• 68 Hydrogenated lanolin, lanolin alcoholの抗原(アレルゲン・抗原)
• 33 腔腸動物イワスナギンチャクの有毒物質パリトキシンおよびその他の成分について
• オートアナライザーによる油脂及び油脂誘導体中の遊離脂肪酸の自動比色定量法

スポンサーリンク