明暗逆転におけるマウスの免疫反応
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概要
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The influence on immune responses of a phase shift in light-dark cycles was studied in mice. Male dd-strain mice weighing 10 to 12g were randomly divided into two groups, and raised for 3 weeks in a climatic chamber kept at 25°C±1°C and 60% rh, with light from 0700 to 1900 hours (LD 12:12). After 3 weeks, one group of mice was transferred into another climatic chamber with a reversed lighting regimen.Mice immunized with the red cells of sheep (6.6×107/g body weight) were sacrified to collect sera and spleen samples in order to investigate the influence of light inversion on primary as well as secondary immune responses, and changes in the course of time of the immune response was studied.Tests for hemagglutination (HA) and hemolytic reaction (HR) were carried out to find the antibody titers in the sera, and the numbers of plaque-forming cells (PFC) in the spleen were counted.The following results were obtained:1) The loss of immune response was significant in mice immunized on the 4th day after inversion of the light-dark cycles with the dark period prolonged, as compared with the control group under an unchanged light-dark regimen.2) On prolonging the light period, similar phenomena were observed.3) The time for the immune response on the 4th day after the inversion of light-dark cycles indicated that antibody production was suppressed only in the initial phase of the immune response.4) Seven days after primary immunization (6.6×107/g body weight), secondary immunization (3.3×107/g body weight) was carried out and the time for the immune response thereafter was investigated. The result indicated that a light inversion exerted little influence on the immune response after secondary immunization.5) By treatment with 2-mercaptoethanol it was found that 19S antibody, and not 7S, was suppressed after the inversion of light-dark cycle in the primary immunization.These results suggest the possibility that the pituitary adrenal axis may play a very important role in immunosuppression after inversion of light-dark cycles in mice.
- 日本衛生学会の論文