Luminol-dependent Chemiluminescenceを応用した血清オプソニン活性の多検体同時測定法 : 第二報測定間の再現性および一山村住民の血清オプソニン活性

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The luminol-dependent chemiluminescence method (LC method) measures the light emitted by luminol through oxidation with reactive oxygen species produced during phagocytosis. Using the LC method technique, we previously succeeded in making a simultaneous multiple measurement system and reported it in this journal.In this paper, we examine the reproducibility of intermeasurement with this system and also report on the serum opsonic activities of inhabitants of a rural area in Oita prefecture.1. For the purpose of producing 4 standard serums graded by the activity of the thermolabile opsonic activator, inactivated serum (56°C, 30min.) and normal serum are mixed in specified proportions. The reproducibility of the standard serums was examined 17 times in 4 months and for each measurement the concentration of the neutrophile suspension and the number of simultaneously measured samples were different.2. Peak time and peak height of chemiluminescence of each standard serum were represented by the ratio calculated from the values of the corresponding standard serum consisting of only pooled normal serum. Good reproducibility was observed in the case of the standard serums which had a large proportion of pooled normal serum.3. In the case of the representation of the pooled normal serum ratio using the least squares method calculated from the measurement values of 4 standard serums, the coefficient of variation of the peak height ranged from 2.0 to 3.1%.4. Serum opsonic activities of 88 inhabitants in a rural area of Oita prefecture were measured 5 times in 2 days by this system and the reproducibility in regard to peak height of standard serums used for these measurements was good.5. In the serums of inhabitants in the rural area, there were some which showed a larger peak height or a shorter peak time than the standard serum consisting of only pooled normal serum, but the mean values were slightly smaller than those of the pooled normal serum.6. The serums were simultaneously measured using the same neutrophil suspension and changes were recognized not only in peak time but also in peak height in each serum. Thus, we concluded that serum opsonic activity is not indicated by peak time alone; it is also necessary to consider peak height as an indicator together with peak time.
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Luminol-dependent Chemiluminescenceを応用した血清オプソニン活性の多検体同時測定法 : 第二報測定間の再現性および一山村住民の血清オプソニン活性

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Luminol-dependent Chemiluminescenceを応用した血清オプソニン活性の多検体同時測定法 : 第二報 測定間の再現性および一山村住民の血清オプソニン活性

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Luminol-dependent Chemiluminescenceを応用した血清オプソニン活性の多検体同時測定法 : 第二報測定間の再現性および一山村住民の血清オプソニン活性