Purification of Human Placental Lactogen
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概要
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In order to obtain a more homogeneous preparation of human placental lactogen (HPL), a proteo-hormone which chiefly possesses lactogenic activity, the extraction and the purification of this protein from human placental tissues was carried out by a modification of the method of Friesen (1965a, b). The extracts of minced placenta were made to be acetone-dried powder. The powder was extracted with water and then the soluble portion was precipitated with half saturated ammonium sulfate. This crude preparation was fractionated on ECTEOLA-cellulose column and Sephadex G-100 equilibrated with 0.05M ammonium bicarbonate, pH8.3. The preparation thus obtained was electrophoretically homogeneous and showed prominent lactogenic effects in the pigeon crop sac assay. No gonadotropic activity of this protein was observed.
- 社団法人 日本内分泌学会の論文
著者
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Kumai Akihiko
Departments Of Endocrinology Medical Research Institute Tokyo Medical And Dental University
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KUMAI AKIHIKO
Department of Chemistry, Research Laboratories, Teikoku Hormone Mfg. Co., Ltd.