Localizations and Biological Activities of HCG Fractions in Rat Organs
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概要
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This study was undertaken to determine which biological site or immunological site in the HCG molecule is essential for uptake of HCG by the ovaries. HCG was obtained from the urine of patients with trophoblastic dieseases by This study was undertaken to determine which biological site or immunological site in the HCG molecule is essential for uptake of HCG by the ovaries. HCG was obtained from the urine of patients with trophoblastic dieseases by adsorption on kaolin. This preparation gave two distinct immunological fractions on Sephadex G-100 column chromatography. One fraction (antigen-1;Ag-1) had both gonadotropic and immunological activities present in HCG, while the other (antigen 2; Ag-2) had only immunological activity of HCG. <SUP>131</SUP>I Ag-1 and <SUP>125</SUP>I Ag-2 were injected into rats intravenously and the localizations of <SUP>131</SUP>I and <SUP>125</SUP>I in each organ and their subcellular fractions were measured by the paired label technique. After injection of HCG, Ag-1 and Ag-2, the activities of Δ<SUP>5</SUP>-3β-hydroxysteroid dehydrogenase (3β-HSD) in the adrenals and ovaries were measured as an index of steroid biosynthesis. The results obtained were as follows:<BR>1. <SUP>131</SUP>I Ag-1 was taken specifically in the ovaries and the ratio of the <SUP>131</SUP>I Ag-1 to <SUP>125</SUP>I Ag-2 in the microsomal fraction was 14:1, 3hours after injection of the mixture containing cold HCG. The heated <SUP>131</SUP>I Ag-1, which possessed only the immunological activity of HCG was not taken specifically in ovaries. These results suggest that the biological site in HCG molecule might be essential for picking up HCG by ovaries.<BR>2. The percentage of <SUP>125</SUP>I Ag-2 localization in kidney gradually increased, but no significant change was found in adrenals, thyroid, uterus, liver and blood.<BR>3. 3β-HSD activity in the ovary was stimulated by the administration of HCG and Ag-1, but slightly inhibited by Ag-2. 3β-HSD activity in the adrenals was clearly inhibited by Ag-2. These results suggest that Ag-2 possibly has some other biological activity.
- 社団法人 日本内分泌学会の論文
著者
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ISOJIMA Shinzo
Department of Obstetrics and Gynecology Hyogo Medical College
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Koyama Koji
Department Of Obstetrics And Gynecology
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Adachi Haruo
Department Of Obstetrics And Gynecology School Of Medicine Tokushima University
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ISOJIMA SHINZO
Department of Obstetrics and Gynecology, School of Medicine, Tokushima University
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Takahashi Hisatosh
Department of Obstetrics and Gynecology, School of Medicine, Tokushima University
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